restriction enzyme

1. Restriction enzyme: Protein ( more specifically, an endonuclease) produced by bacteria that cleaves DNA at specific sites along its length.
   限制性内切：由细菌产生的一种蛋白质，能在特定的地方切断去氧核糖核酸分子。
2. When genome transplantations were performed using the restriction enzyme minus recipient cells, all the genome transplantations worked regardless of if the DNA was methylated or not.
   结果显示不论DNA甲基化与否，所有使用受体细胞限制性内切酶的基因组移植都获得成功。
3. By restriction enzyme identification, PCR and sequence analysis, the recombinant plasmid of the HA1 was successfully constructed.
   结果经双酶切、PCR及测序鉴定证实血凝素基因HA1区的真核表达载体构建成功。
4. Restriction Enzyme Mediated Integration, a New Strategy for Cloning New Genes
   限制酶介导整合技术：一种克隆新基因的新策略
5. Results The expressive vector has been constructed and confirmed by restriction enzyme digestion and DNA sequencing analysis.
   结果构建的表达载体经过限制性内切酶分析和DNA序列测定，证实准确无误。
6. Some recognization about the checking method in kind in the thorough examination of the grain warehouse The Restriction Enzyme Database from NEB.
   粮食清仓查库实物检查方法几点认识限制莓资料库取自新英格兰生物实验室公司。
7. It enables a specific gene to be located on a particular restriction enzyme fragment.
   它就能使专一的基因被定位于特定的限制性内切酶切成的片段上。
8. Taken together, PCR-RFLP, based on PCR and DNA restriction enzyme digestion, can quickly and accurately identify cryptic pollinating-fig wasp species.
   我们的结果表明基于PCR和DNA酶切技术能快速而准确地区分传粉榕小蜂的隐种。
9. Rapid site-directed mutagenesis on full-length plasmid DNA by using designed restriction enzyme assisted mutagenesis
   用DREAM技术进行全长质粒快速定点突变
10. Antisense RNA of survivin was confirmed by restriction enzyme digestion and DNA sequencing.
    经限制性酶切和测序鉴定所构建的反义核酸是否正确；
11. This is the website of the Restriction Enzyme Database.
    这是限制酶数据库的网站。
12. Zinc finger nucleases ( ZFNs) are artificial restriction enzymes made by fusing an engineered zinc finger DNA-binding domain to the DNA cleavage domain of a restriction enzyme.
    锌指核酸酶是一种人工制做限制性内切酶，通过将锌指DNA结合区与限制性内切酶的DNA切割区融合获得。
13. The DNA vaccine was identified by restriction enzyme analysis, sequencing and expression.
    对此疫苗进行酶切、测序和表达鉴定。
14. The length and the results of restriction enzyme digestion indicate that the amplified products are respected.
    各扩增产物长度和限制性酶酶切结果表明均为预期产物。
15. The mutation was confirmed by digestion of the PCR products with restriction enzyme.
    发现突变后用特异性限制性内切酶对突变位点进行酶切，验证突变。
16. Its specificity was tested by direct PCR, a complementation, restriction enzyme cleavage, and sequencing method.
    联合应用PCR鉴定、α互补法、限制性酶切和序列分析法鉴定其特异性。
17. Restriction enzyme mapping and SDS-PAGE protein analysis were done on four transformants.
    还进行了限制酶酶切分析和蛋白质SDS-PAGE分析。
18. The genomic DNAs prepared by both CTAB and the modified SDS were suitable for digestion with restriction enzyme.
    CTAB法和SDS改进法所得DNA都可直接应用于限制性酶切分析；
19. The DNA was suitable for digesting with restriction enzyme and Random Amplified Polymorphic DNA reaction without purified with RNase.
    提取的DNA无需经RNase处理，可直接用于限制性内切酶酶切和随机扩增多态DNA反应。
20. Dot blotting and restriction enzyme digestion analysis showed recombinant pDBT containing three genes was obtained.
    通过点杂交和酶切鉴定，得到了带有3个抗性基因的双抗植物表达载体pDBT。
21. The result of PCR-restriction enzyme digestion was identical with that of DGGE.
    PCR-限制性酶切结果与DGGE所测结果完全吻合。
22. These two fragments were cloned into the pMD-18T vector for the partial sequencing and restriction enzyme analysis.
    将扩增产物克隆到pMD-18T上，经酶切分析、部分序列测定和同源比较确认得到了所需的基因片段。
23. The recombinant plasmid of pGTE-TK was identified by restriction enzyme analysis and sequencing, which proved completely its validity.
    对重组质粒进行限制性内切酶分析和基因测序，证实了克隆片段的可靠性。
24. Both genes were correctly cloned and identified by PCR, restriction enzyme digestion and sequencing.
    经PCR鉴定、酶切鉴定和测序说明所克隆的两种基因是正确的。
25. And have been identified by PCR and restriction enzyme digestion successfully constructed recombinant viruses.
    并经过PCR和酶切鉴定重组病毒构建成功。
26. The construct was confirmed by PCR, restriction enzyme digestion and direct sequencing.
    经限制性内切酶酶切和测序证实。
27. To verified the by restriction enzyme digestion, bacterial colony PCR, and sequencing analysis.
    并对重组质粒进行双酶切、菌落PCR及测序分析进行鉴定。
28. The target fragment was digested by restriction enzyme to determine the accuracy of the detection technology.
    用限制性内切酶酶切的方法判断该检测技术的准确性。

noun

1. any of the enzymes that cut nucleic acid at specific restriction sites and produce restriction fragments

   Synonym:    restriction endonucleaserestriction nuclease